Table 3.
RealTime-PCR analysis of selected genes1
| Locus | Description and putative function | UA159 | TW26D | Fold-difference | Parametric P-value |
|---|---|---|---|---|---|
| Planktonic | |||||
| smu.1128 | Sensor/histidine kinase, CiaH | 8.17E5(1.13E4) | 2.62E5(1.93E4) | −3.12 | 0.01538 |
| smu.610 | Surface antigen SpaP | 1.39E6(1.06E5) | 3.98E5(2.89E4) | −3.49 | 0.00172 |
| smu.987 | Surface protein WapA | 4.52E6(1.54E6) | 5.91E6(2.1E6) | 1.32 | 0.02389 |
| smu.872 | Fructose-PTS, EllABCFru | 5.39E7(3.0E7) | 1.69E6(1.9E6) | −31.8 | 6.74E-07 |
| smu.78 | Exo-β-fructosidase, FruA | 4.63E5(1.79E4) | 1.98E5(7.66E3) | −2.34 | 6.45E-04 |
| smu.985 | β-glucosidase, BglA | 9.88E4(3.1E3) | 8.7E4(2.5E4) | −1.14 | 0.02897 |
| smu.364 | Putative glutamine synthetase | 3.59E7(2.03E6) | 2.53E7(3.5E6) | −1.43 | 0.04326 |
| smu.1877 | Mannose-PTS, EllDMan | 4.72E6(8.7E5) | 2.00E6(2.6E5) | −2.36 | 0.00869 |
| Biofilms | |||||
| smu.44 | DNA mismatch repair protein | 6.22E5(4.9E4) | 1.13E5(4.8E4) | −5.5 | 7.85E-04 |
| smu.45 | DNA mismatch repair protein | 2.44E5(1.3E4) | 1.32E4(1.9E3) | −18.48 | 5.60E-04 |
| smu.46 | LuxR-type regulator | 4.95E5(4.2E4) | 7.21E3(1.3E3) | −68.65 | 3.43E-04 |
| smu.47 | NHN-type endonuclease | 1.49E7(6.8E6) | 1.93E5(2.7E4) | −77.2 | 5.11E-04 |
| smu.299 | Bacteriocin precursor | 8.84E6(7.1E5) | 5.84E6(1.3E6) | −1.52 | 2.59E-04 |
| smu.423 | Mutacin-like protein | 1.60E5(1.1E5) | 8.77E5(1.1E5) | 5.48 | 2.38E-04 |
| smu.985 | β-glucosidase BglA | 2.27E5(9.2E4) | 4.2E5(4.4E4) | 1.85 | 4.51E-03 |
| smu.1528 | F-ATPase, β-subunit | 7.5E6(3.8E5) | 1.37E7(7.5E6) | 1.82 | 6.74E-03 |
| smu.1877 | Mannose-PTS, EllDMan | 3.55E6(3.38E5) | 1.02E6(4.0E5) | −3.48 | 2.67E-04 |
| smu.1961 | Fructose/mannose-PTS, LevD | 1.41E6(1.01E6) | 3.19E6(1.9E6) | 2.26 | 5.54E-03 |
| 16S-1 rRNA | 16S rRNA | 2.16E7(3.5E6) | 2.19E7(5.1E6) | 0.99 | 0.45 |
1
Results are expressed as copy numbers in 1 μg of total RNA. The data represent mean (± standard deviation) of no less than four independent experiments. See Table 2 for more information.