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. 2011 May 30;193(5):885–900. doi: 10.1083/jcb.201012083

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© 2011 Saraya et al.

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Figure 5. — Artificial targeting of Pex3 to the ER does not restore peroxisome formation in the absence of Pex25. P_AOX BIP_N30PEX3-mCherry was introduced in pex3 cells (A), pex3 pex11 cells (B), pex3 pex25 cells (C), or pex3 pex11 pex25 cells (D). Electron microsopy analysis of cells grown for 16 h on glycerol/methanol-containing media failed to resolve peroxisomal structures in cells lacking Pex25. N, nucleus; P, peroxisome; V, vacuole. Bar, 0.5 µm. The asterisk represents a cytosolic alcohol oxidase crystalloid.