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. 2011 May 30;193(5):885–900. doi: 10.1083/jcb.201012083

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© 2011 Saraya et al.

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Figure 9. — Identification and localization of Rho1. Rho1 was identified through a screen for conditional mutants affected in the formation of peroxisomal structures in H. polymorpha pex3 cells that produce the first 50 amino acids of the PEX3 gene fused to GFP(N_50.Pex3-GFP). (A) Fluorescence microscopy images of methanol/methylamine-grown cells of the pex3 P_AMON₅₀PEX3-GFP control strain grown at 35°C (A) or 44°C (B) and pex3 RHO1^tsP_AMO N₅₀PEX3-GFP grown at 35°C (C) or 44°C (D). (E) Localization of GFP-Rho1, produced in WT H. polymorpha cells that also synthesize DsRed-SKL to mark peroxisomes. (F–I) Pex25-mCherry fluorescence in pex11 pex25 P_AOX PEX25-mCherry cells grown at at 35°C (F) or 44°C (G) and in pex25 pex11 RHO1^ts P_AOX PEX25-mCherry cells upon cultivation at 35°C (H) and 44°C (I). Bar, 1 µm.