Fig. 2.

Sinigrin hydrolysis and urinary excretion of AITC. Groups of five female F344 rats were administered a single oral dose of sinigrin, MSP-1 or AITC. Both sinigrin and MSP-1 were mixed in water, whereas AITC was mixed in soy oil, which were given to rats within 30 min of preparation. The rats were kept in metabolism cage for 24 h urine collection (1 rat per cage). Urinary levels of ITC equivalents were measured by the cyclocondensation assay. All values were adjusted by background urinary levels of ITC equivalents, which were 8.6 ± 2.3 μM (average 24 h urinary concentration) and 0.05 ± 0.01 μmol (24 h urine), determined in another group of rats. Each value is mean ± SEM (n = 5).