Figure 2.

The contrast agent (Optison) enhanced the ability of Doppler sonography to depict microscopic ovarian tumor-associated vasculature in laying hens. A, Precontrast sonogram of an ovary without a detectable solid ovarian mass or abnormality. Only a few blood vessels are shown in this ovary, with no preovulatory follicle. B, Corresponding sonogram of the same ovary showing the arrival of Optison. Compared to the precontrast image, the number of detectable blood vessels is increased, and the vessels appear more dilated in the postcontrast sonogram. C, Postcontrast sonogram of the same ovary showing the peak level of enhancement. Compared to the precontrast image and postcontrast image at the arrival of Optison, more vessels are shown at peak enhancement. Although no solid ovarian mass is shown, the central vascular arrangement pattern indicates a potential ovarian abnormality. D, Gross appearance of the same ovary at euthanasia. As predicted, neither a large preovulatory follicle nor a detectable solid mass is shown. However, subsequent histopathologic examination showed the presence of an endometrioid lesion (termed a microscopic ovarian tumor), confirming the contrast-enhanced sonographic prediction. Scanning of hens and their subsequent processing were similar to those mentioned in Figure 1. Dotted circle indicates the ovary; and S, stroma.