Table 1. CARD-FISH probes used in this study. Percent formamide in hybridization buffer and NaCl concentration in wash buffer are noted for each probe.
| Probe | Specificity | % FA | Concentration NaCl in wash buffer in Mol |
|---|---|---|---|
| EUB338-I-III | Most bacteria | 35 | 0.080 |
| NON 338 | Background control | 35 | 0.080 |
| ARCH915 | Most archaea | 35 | 0.080 |
| ALF968 | 81% Alpha- proteobacteria | 35 | 0.080 |
| GAM42a | Most gamma- proteobactera | 35 | 0.080 |
| GAM42a competitor | 35 | 0.080 | |
| BET42a | Most beta- proteobactera | 35 | 0.080 |
| BET42a competitor | 35 | 0.080 | |
| DELTA495a, b and c | Most delta- proteobacteria | 35 | 0.080 |
| cDELTA495a, b and c | 35 | 0.080 | |
| EPS549 | Most epsilon- proteobacteria | 55 | 0.020 |
| ARC94 | Arcobacter | 20 | 0.225 |
Abbreviations: CARD-FISH, Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization; FA, formamide.