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Schematic illustration of reinfection/sporulation assay. (a): wild type bacteria infect cured fungi, migrate into sporangia, trigger formation of sporangiospores and enter the spores. (b): T3SS mutants enter hyphae, but reach lower cell densities and fail to elicit sporulation. (c): cured fungi are incapable of forming mature sporangia and sporangiospores. Premature sporangia can be detected sporadically.
