Figure 3.

Assembly of 1825 reads (out of 2259 total) binning to the P. ubique HTCC1002 proteorhodopsin gene PU1002_03206 (left), and of 10 879 reads (out of 10 879 total) binning to the internal transcript standard (right). (a) Percent nucleotide divergence from the consensus sequence. (b) Percent nucleotide divergence from the reference sequence. (c) Coverage by nucleotide position. (d) Read assembly to the reference gene (shown in red), with dashed lines indicating start and end positions of the reference. Note that the reference gene lengths are extended by assembly gaps. Divergence from the consensus sequence (that is, the majority nucleotide at a given position) is indicated as follows: A= red, T= green, C= blue and G= yellow. Insets show close-up regions of assemblies.