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. 2010 Oct 21;5(3):486–496. doi: 10.1038/ismej.2010.146

Figure 2.

Figure 2

Gas chromatograms showing the degradation of four BPBA isomers over a 49-day incubation period by an enrichment culture. Enrichments grown on n-BPBA (a), iso-BPBA (b), sec-BPBA (c) or tert-BPBA (d). Clearly visible is the disappearance of the n-BPBA trimethylsilyl (TMS) ester peak (RT: 17.70) and the appearance of a secondary peak at 14 days (RT: 15.20), confirmed as n-BPEA (TMS ester). This is further degraded within 49 days with the transient production of another peak (RT: 19.67), tentatively assigned as the bis-TMS ester of (4′-carboxybutylphenyl)ethanoic acid. Clearly visible is the disappearance of the iso-, sec- and tert-BPBA trimethylsilyl (TMS) ester peak and the appearance of another peak in 14 days, confirmed as iso-, sec- and tert-BPEA (TMS ester) respectively. This is not further transformed during the course of the experiment (49 days).