Table 1. PCR primers used in SAG screens and clone library construction.
| Forward primer | Reverse primer | Region [bp]a | References |
|---|---|---|---|
| Euk1A 5′-CTGGTTGATCCTGCCAG-3′ | 516r 5′-ACCAGACTTGCCCTCC-3′ | 4–563 | Amann et al., 1990; Sogin and Gunderson, 1987 |
| 528f 5′-CCGCGGTATTCCAGCTC-3′ | EukB 5′-TGATCCTTCTGCAGGTTCACCTAC-3′ | 572–1795 | Elwood et al., 1985; Medlin et al., 1988 |
| Euk1A 5′-CTGGTTGATCCTGCCAG-3′ | EukB 5′-TGATCCTTCTGCAGGTTCACCTAC-3′ | 4–1795 | Amann et al., 1990; Medlin et al., 1988 |
| 515f 5′-GTGCCAAGCAGCCGCGGTAA-3′ | 1209r 5′-GGGCATCACAGACCTG-3′ | 515–1209 | Giovannoni et al., 1988; Reysenbach et al., 1992 |
| 345f 5′-AAGGAAGGCAGCAGGCG-3′ | 499r 5′-CACCAGACTTGCCCTCYAAT-3′ | 345–499 | Zhu et al., 2005 |
a
Region targeted by PCR on Saccharomyces cerevisiae (accession number AY251629.1) 18S rRNA gene.