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. 2010 Nov 25;5(4):717–727. doi: 10.1038/ismej.2010.173

Figure 5.

Figure 5

C and O 1s NEXAFS spectra of M. ferrooxydans culture (stalk, cells and surrounding minerals), Loihi microbial mat stalk, and standards. Dashed lines indicate the following energies: 285.2, 287.3, 288.2, 288.6 and 289.3 e (see Table 1); 529.9, 531.3 and 532.1 e (O 1s). C standards include E. coli lipopolysaccharide (LPS), bovine serum albumin (protein), alginate (acidic polysaccharide), agarose (neutral polysaccharide), DNA and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (phospholipid). FeOOH standards (phase confirmed by X-ray diffraction) include lepidocrocite, two-line ferrihydrite, akaganeite and goethite. Iron(III) oxyhydroxides exhibit transitions occurring from both O 1s (peaks a and b) and OH 1s (peak c) core orbitals.