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. 2010 Dec 2;5(5):810–821. doi: 10.1038/ismej.2010.183

Table 2. Properties of sequences used to test primer and probe specificity.

Target sequence Closest relative Base-pair mismatches for closest relative
Ct from 107 target molecules Ct from 107 non-target molecules
    Percent nucleotide identity Forward primer Reverse primer Probe    
LO.16jul08.14 LO1a-34 77 7 5 5 14.77 No Ct
LO.16jul08.20 LO1a-68 81 2 4 4 13.92 31.49
LO.20may09.33 UTM.09jun09.47 69 6 7 13 12.34 33.14
LO.08may08.08 UTM.21sept09.07 76 6 7 4 12.30 No Ct