Table 2. Properties of sequences used to test primer and probe specificity.
Target sequence | Closest relative |
Base-pair mismatches for closest relative |
Ct from 107 target molecules | Ct from 107 non-target molecules | |||
---|---|---|---|---|---|---|---|
Percent nucleotide identity | Forward primer | Reverse primer | Probe | ||||
LO.16jul08.14 | LO1a-34 | 77 | 7 | 5 | 5 | 14.77 | No Ct |
LO.16jul08.20 | LO1a-68 | 81 | 2 | 4 | 4 | 13.92 | 31.49 |
LO.20may09.33 | UTM.09jun09.47 | 69 | 6 | 7 | 13 | 12.34 | 33.14 |
LO.08may08.08 | UTM.21sept09.07 | 76 | 6 | 7 | 4 | 12.30 | No Ct |