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. 2010 Dec 2;5(5):801–809. doi: 10.1038/ismej.2010.177

Table 1. Primers used in this study.

Name Sequence (5′-3′) Specificity Reference
4Fa TCCGGTTGATCCTGCCRG Archaea Giovannoni et al., 1988
27Fa AGAGTTTGATCMTGGCTCAG Bacteria Lane, 1990
T7-1492Ra,b TCTAATACGACTCACTATAGGGGGYTACCTTGTTACGACTT Universal  
MitoFa TACTACCAGACAACCTTAGC Mitochondrial 16S Kasashima et al., 2008
T7-MitoRa,b TCTAATACGACTCACTATAGGGGTTTCGGGGGTCTTAGCTTT    
StrF CGACGATACATAGCCGACCTGAG streptococci Chalmers et al., 2008
StrR TCCATTGCCGAAGATTCCCTACTG    
F-Ent ATGGCTGTCGTCAGCTCGT enterobacteria Leser et al., 2002
R-Ent CCTACTTCTTTTGCAACCCACTC   Sghir et al., 2000
EcocF CCCTTATTGTTAGTTGCCATCATT enterococci Rinttila et al., 2004
EcocR ACTCGTTGTACTTCCCATTGT    
BacF CGGCAACGAGCGCAACCC Bacteria Denman and McSweeney, 2006
BacR CCATTGTAGCACGTGTGTAGCC    
Fprau420R CCGAAGACCTTCTTCCTCC F. prausnitzii Wang et al., 1996
Fprau223F GATGGCCTCGCGTCCGATTAG   Ahmed et al., 2007
a

Primers used for preparation of target RNA for array hybridization.

b

Modified by Kang et al. (2010) to include the T7 promoter (italicized) for subsequent cRNA synthesis.