Table 1. Primers used in this study.
Name | Sequence (5′-3′) | Specificity | Reference |
---|---|---|---|
4Fa | TCCGGTTGATCCTGCCRG | Archaea | Giovannoni et al., 1988 |
27Fa | AGAGTTTGATCMTGGCTCAG | Bacteria | Lane, 1990 |
T7-1492Ra,b | TCTAATACGACTCACTATAGGGGGYTACCTTGTTACGACTT | Universal | |
MitoFa | TACTACCAGACAACCTTAGC | Mitochondrial 16S | Kasashima et al., 2008 |
T7-MitoRa,b | TCTAATACGACTCACTATAGGGGTTTCGGGGGTCTTAGCTTT | ||
StrF | CGACGATACATAGCCGACCTGAG | streptococci | Chalmers et al., 2008 |
StrR | TCCATTGCCGAAGATTCCCTACTG | ||
F-Ent | ATGGCTGTCGTCAGCTCGT | enterobacteria | Leser et al., 2002 |
R-Ent | CCTACTTCTTTTGCAACCCACTC | Sghir et al., 2000 | |
EcocF | CCCTTATTGTTAGTTGCCATCATT | enterococci | Rinttila et al., 2004 |
EcocR | ACTCGTTGTACTTCCCATTGT | ||
BacF | CGGCAACGAGCGCAACCC | Bacteria | Denman and McSweeney, 2006 |
BacR | CCATTGTAGCACGTGTGTAGCC | ||
Fprau420R | CCGAAGACCTTCTTCCTCC | F. prausnitzii | Wang et al., 1996 |
Fprau223F | GATGGCCTCGCGTCCGATTAG | Ahmed et al., 2007 |
Primers used for preparation of target RNA for array hybridization.
Modified by Kang et al. (2010) to include the T7 promoter (italicized) for subsequent cRNA synthesis.