Table 2. Oligonucleotide probes for FISH analysis and primers for PCR-DGGE and qPCR analysis in this study.

Code	Function	Sequence (5′–3′)	Specificity	Reference
EUB338	Probe	gctgcctcccgtaggagt	Bacteria	Amann et al. (1990)
EUB338II	Probe	gcagccacccgtaggtgt	Bacteria	Daims et al. (1999)
EUB338III	Probe	gctgccacccgtaggtgt	Bacteria	Daims et al. (1999)
UCB823	Probe	cctccccaccgtccagtt	P. acidivorans	Johnson et al. (2009a)
Beta42a	Probe	gccttcccacttcgttt	Betaproteobacteria	Manz et al. (1992)
Gama42a	Probe	gccttcccacatcgttt	Gammaproteobacteria	Manz et al. (1992)
ZAL	Probe	cttccatactctaggtac	Zoogloea	Rossello-Mora et al. (1995)
ZRA	Probe	ctgccgtactctagttat	Zoogloea	Rossello-Mora et al. (1995)
ZBE	Probe	tgccaaactctagccttg	Zoogloea	Rossello-Mora et al. (1995)
341F-GC	Primer	cctacgggaggcagcaga	Bacteria	Schäfer and Muyzer (2001)
341F	Primer	cctacgggaggcagcag	Bacteria	Schäfer and Muyzer (2001)
907R	Primer	ccgtcaattcmtttgagttt	Bacteria	Schäfer and Muyzer (2001)
UCB127F	Primer	gaatctgccccacgggtg	P. acidivorans	This study
UCB823R	Primer	cctccccaccgtccagtt	P. acidivorans	This study

Abbreviations: FISH, fluorescence in situ hybridization; DGGE, denaturing gradient gel electrophoresis; q-PCR, quantitative polymerase chain reaction.

^a341F-GC primer contains GC-clamp (cgcccgccgcgccccgcgcccgtcccgccgcccccgcccg) at the 5′-end of the primer.