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(A) Concentration−response curve of the apoptosis-mediated total cell death by digitoxin analogues in 12 h treatment. (B) Apoptotic cell death percentage was compared for each compound at 50 nM concentration (one-way ANOVA; ***, P < 0.001). (C) Hoechst-stained apoptotic cells appear in blue, and propidium iodide-stained necrotic cells appear in red at 50 nM for compounds 1, 3, 6, 7, DIGOH.
