Table 1.
Approximate Yield from Each Depletion Method
| Method | Load Volume (μl) | Load (μg) | Yield (μg) | Yield % | Yield Concentration (μg/μl) |
|---|---|---|---|---|---|
| Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich) | 25 | 1500 | 216 | 14.39 | 1.727 |
| Multiple Affinity Removal System (5185, Agilent) | 20 | 1200 | 133 | 11.08 | 2.871* |
| Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich) | 50 | 3000 | 340 | 11.34 | 1.513 |
*The protein concentration in the depleted solution of Multiple Affinity Removal System (Agilent) was detected after concentration by ultrafiltration using a 5000 Da molecular mass cut-off centrifugal concentrator.