Table 1.
Top 20 high frequency sequences in raw sequencing data
| read sequence | frequencies | genome presence | |||||
|---|---|---|---|---|---|---|---|
| rank | total | 1.fq | 2.fq | Ori1 | Pos2 | Junction3 | |
| GCTCTTCGGAAAGGTCAAAAACAGTTTGAG...... | 1 | 828 | 427 | 401 | F | 30641 | tctatttggagctcttcgga |
| GTTTTACAGAATCGTACTCGGCCTTGTTCG...... | 2 | 705 | 388 | 317 | F | 3272 | aattactggagttttacaga |
| GTATAATGATTCATCAACAAACAAAAGACA...... | 3 | 692 | 383 | 309 | R | 30486 | cccttttggagtataatgat |
| GCGTAATTCCACCTTTTTCTTCCCAATCTT...... | 4 | 673 | 352 | 321 | F | 52555 | tcttgttggagcgtaattcc |
| GGTATACATCATTAAATAACGATGTATATC...... | 5 | 641 | 333 | 308 | F | 163251 | agaaattggaggtatacatc |
| GTATTTCAAGAAACGTGATAAAGCCCAGGC...... | 6 | 577 | 318 | 259 | F | 121764 | aacgtttggagtatttcaag |
| GCGTAATTGCTTCAGGTAAGCCTTTAGGAT...... | 7 | 505 | 256 | 249 | F | 73140 | agaatatggagcgtaattgc |
| GTGCATGATTGGTAACAGTTCGGCAACCCA...... | 8 | 505 | 277 | 228 | F | 40411 | ggtctttggagtgcatgatt |
| GTTTTACAGACAACGCAAATCTTATCTGAC...... | 9 | 496 | 253 | 243 | F | 115803 | atcgattggagttttacaga |
| GCTGAAAAGGCAGCTGAAACTAAAGCCGCT...... | 10 | 494 | 270 | 224 | R | 3702 | taaattagcagctgaaaagg |
| GTATAATGTAAAAACAAACCTGAGGAAATT...... | 11 | 490 | 274 | 216 | R | 32654 | ctcccttggagtataatgta |
| GTATTAACAAGATTCCAGAATTTCTCACCC...... | 12 | 481 | 253 | 228 | R | 75276 | gttttctggagtattaacaa |
| GTTTCTCAGCGATTTTAATCGACCACTCTT...... | 13 | 448 | 238 | 210 | F | 29924 | tcgtcttggagtttctcagc |
| GTTACATAAGCATCAGGAGCAGATGGTCCC...... | 14 | 445 | 254 | 191 | R | 102003 | ttgctttggagttacataag |
| GCTTTAATCTTAACAATAGTGCCGAGATAA...... | 15 | 443 | 245 | 198 | F | 165136 | gtatttacctgctttaatct |
| GCTGAACGTACCGAAGTTGCAGGTATGACT...... | 16 | 440 | 266 | 174 | R | 28799 | gttgttcagagctgaacgta |
| GTATAATCTTTCTATCAACTTGAGGAGAAT...... | 17 | 434 | 217 | 217 | R | 46215 | gatggatggagtataatctt |
| GCTGCATCTTCAGATTGGTCTTCGTCTTCA...... | 18 | 431 | 251 | 180 | F | 5448 | ttcagatggagctgcatctt |
| GTTATTACTAAACAAGTTTTTAACCGCACT...... | 19 | 426 | 222 | 204 | R | 122567 | ctcccttggagttattacta |
| GTTAACAAATGCCATACGACATTTAAGGGA...... | 20 | 425 | 208 | 217 | F | 56968 | aacgtttagagttaacaaat |
1 Orientation in genome. F, forward; R, reverse.
2 Position in genome.
3 The genomic sequence around the start site of the HFSs.