Figure 5. Effect of PKC inhibitors on THP-1 cells synthesis of proMMP-9/CSPG heteromer and proMMP-9.

Shown is a typical experiment where cells in the absence (−) and presence (+) of PMA (10⁻⁷ M) were incubated with various concentrations of the PKC inhibitors Gö6976 and Gö6983 (A, B) and Rottlerin (C, D). In the presence of Gö6976 and Gö6983 (A, B), cells were incubated for 72 h, while in the presence of Rottlerin (C, D) the cells were incubated for 12 h in serum free medium. To detect the effect of the PKC inhibitors on the synthesis of the proMMP-9/CSPG heteromer (A, C), the harvested media was applied to Q-Sepharose chromatography as described in Materials and Methods prior to gelatin zymography. To determine the effect of the inhibitors on the synthesised proMMP-9 (B, D), the harvested medium was diluted 20 times and then applied to gelatin zymography. Arrowhead shows the border between the separating and stacking gel and the position of purified proMMP-9 monomer (92 kDa) and proMMP-9 homodimer (225 kDa) used as a standard (Std) is shown.