Figure 5.

Interferon (IFN) γ production from lymph node cells and Borrelia burgdorferi outer-surface protein A (OspA) antibody titers in OspA-vaccinated mice with the DR4 or DR11 transgene lacking the CD28 co-receptor. DR11-tg and DR4-tg mice (15 mice per group) were vaccinated in the footpad (50 μg per mouse) with recombinant OspA (rOspA) in Freund's complete adjuvant. A, production of IFN-γ after in vitro restimulation of popliteal lymph node cells with rOspA (10 μg/mL) was assessed by enzyme-linked immunosorbent assay (ELISA). B, anti-OspA antibody titers were measured by ELISA 2 weeks after OspA vaccination. In both panels, mean values (± standard error of the mean) are given and include the results of 4 or 5 independent experiments. For the comparison in panel A, P = .003. For the comparison in panel B, P = .005. Reproduced with permission from Iliopoulou et al [29].