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. Author manuscript; available in PMC: 2011 Jun 2.
Published in final edited form as: Anal Biochem. 2010 Feb 1;400(2):251–258. doi: 10.1016/j.ab.2010.01.037

Fig. 2.

Fig. 2

Variability in ELISA detection of specific IgG by MDI–albumin conjugates. MDI–albumin conjugates generated with different amounts of MDI, ranging from 0 to 1000 μg MDI/mg albumin, were used (as antigens) to coat microtiter plates in an ELISA for human IgG binding using serum samples from 10 MDI-exposed subjects and 2 representative unexposed individuals. All serum samples were diluted 1:200. The y axis reflects the amount of specific IgG binding. All samples were tested in duplicate, and the results shown are representative of three independent experiments.