Fig. 3.
Separation of MDI-conjugated (albumin) peptides by HPLC. Control (red) or MDI-conjugated (blue) albumin was trypsin digested and fractionated by HPLC as described in Materials and methods. The absorbance of the column elution at 245 nm (y axis) is plotted over time in minutes (x axis). Note different scales for the MDI–albumin samples (left axis) versus the control albumin samples (right axis). Data shown are representative of four independent experiments. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)