Figure 4.

TEM identification and quantitation of autophagosomes in Purkinje cells of normal-fed and food-restricted mice. (A) Images from Purkinje cells of normal-fed (left) or 48 hour food-restricted (middle & right) mice are shown. (B) A single Purkinje cell from a 48 hour food-restricted mouse is shown. Autophagosomes are enclosed in colored boxes, and higher-magnification images of each are provided, with autophagosomes indicated by white arrows. For both normal-fed and food-restricted mice, autophagosomes were enumerated in TEM sections of ∼20 different Purkinje cells; sections from food-restricted mice showed a ∼4-fold increase in autophagosome number (graph, bottom right; p < 0.0002 by Student's t test).