. Author manuscript; available in PMC: 2011 Jun 2.

Published in final edited form as: Xenobiotica. 2009 Apr;39(4):312–322. doi: 10.1080/00498250802714915

Table 2.

Kinetic parameters for flavonoid sulfation catalysed by hSULT1A1.

Flavonoid	V_max (nmol mg⁻¹ min⁻¹)	K_m (μM)	V_max/K_m (ml mg⁻¹ min⁻¹)	K_i (μM)
Catechin	187 ± 9.9	165 ± 8.1	1.14 ± 0.01	6.37 ± 0.22
Epicatechin	188 ± 53	301 ± 89.0	0.62 ± 0.01	8.7 ± 3.07
Hesperetin	92.7 ± 2.3	63.0 ± 0.6	1.47 ± 0.02	3.7 ± 0.11
Eriodictyol	98.6 ± 2.5	80.8 ± 3.3	1.22 ± 0.03	6.1 ± 0.14

Notes: Data are the means ± standard deviation (SD) of three determinations.

Kinetic parameters were estimated using equation (2) (see the Materials and methods section).