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. Author manuscript; available in PMC: 2011 Jun 2.
Published in final edited form as: Xenobiotica. 2009 Apr;39(4):312–322. doi: 10.1080/00498250802714915

Table 3.

Kinetic parameters for flavonoid sulfation catalysed by hSULT1A3a.

Flavonoid Vmax (nmol
mg−1 min−1)
Km (μM) Vmax/Km (ml
mg−1 min−1)
Ki (μM)
Catechin 342 ± 8.2 236 ± 5.6 1.45 ± 0.02 8.0 ± 0.29
Epicatechin 442 ± 9.7 437 ± 10.1 1.01 ± 1.02 9.5 ± 0.21
Hesperetin 303 ± 5.8 179 ± 3.7 1.69 ± 0.02 4.2 ± 0.06
Eriodictyol 269 ± 5.2 193 ± 3.3 1.39 ± 0.03 4.6 ± 0.02

Notes: Data are the means ± standard deviation (SD) of three determinations.

Kinetic parameters were estimated using equation (2) (see the Materials and methods section).