Table 4.
Inhibition constants (IC50) of dietary flavonoid inhibition of recombinant and human intestine cytosolic SULTs.
| Flavonoid | 2-Naphthol sulfation |
Dopamine sulfation by hSULT1A3 |
Oestradiol sulfation |
DHEA sulfation |
||||
|---|---|---|---|---|---|---|---|---|
| hSULT1A1 | Cytosol | hSULT1E1 | hSULT1A1 | Cytosol | hSULT2A1 | Cytosol | ||
| Flavanones | ||||||||
| Hesperetin | 23.4 ± 5.75 | 32.2 ± 3.6 | 20.8 ± 8.1 | 90.4 ± 11.5 | n.a. | 3.6 ± 0.4 | 152 ± 14.9 | 91.0 ± 9.5 |
| Eriodictyol | 15.2 ± 0.9 | 28.9 ± 4.1 | 14.7 ± 8.9 | 677 ± 120.3 | n.a. | 11.8 ± 2.0 | 139 ± 8.8 | 66.1 ± 20.2 |
| Flavonols | ||||||||
| Catechin | 8.4 ± 3.3 | 67.1 ± 6.3 | 82.5 ± 31.5 | n.a. | 2.6 ± 0.4 | 10.1 ± 1.7 | n.a. | 13.6 ± 2.6 |
| Epicatechin | 42.5 ± 8.5 | 157 ± 37.6 | 117 ± 12.9 | n.a. | 6.1 ± 1.0 | 22.7 ± 5.6 | n.a. | 88.4 ± 46.0 |
Notes: Substrates used were as follows: 100 μM 2-naphthol, 50 μM dopamine, 2 μM DHEA, and 0.15 μM oestradiol. IC50 (μM) was calculated using SigmaPlot 9.01. Results are means ± standard deviation (SD) of three determinations.
n.a., Not applicable.