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. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2011 Feb 4;252(2):150–158. doi: 10.1016/j.taap.2011.01.020

Figure 5.

Figure 5

TCDD-inducible and concentration-dependent DNA binding at Prdm1 intron 5 MARE. (A) Electrophoretic mobility shift assay performed on naive and LPS-activated (10 μg/ml) B cells treated with TCDD (10 nM) or DMSO (0.02%) for 0 or 4 h. The 0 h indicates background binding in untreated B cells. (B) Electrophoretic mobility shift assay performed on LPS-activated (10 μg/ml) B cells treated with the indicated concentrations of TCDD or 0.02% DMSO for 4 h. In both assays, binding reactions were set up with 32P-labeled intron 5 MARE probe as described in experimental methods. The arrow head indicates the TCDD-inducible DNA-protein complex. Results are representative of three separate experiments.