Skip to main content
. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2011 Feb 4;252(2):150–158. doi: 10.1016/j.taap.2011.01.020

Figure 6.

Figure 6

Supershift analysis of TCDD-inducible binding activity of Bach2 at Prdm1 promoter and intron 5 MARE. Electrophoretic mobility shift assays were performed on resting and LPS-activated (10 μg/ml) B cells treated for 4 h with TCDD (10 nM) or vehicle (0.02% DMSO). Binding reactions were set up with (A) 32P-labeled promoter MARE probe and (B) 32P-labeled intron 5 MARE probe. In both assays, anti-Bach2 antibody at 1 μg and 2 μg (lanes 5 and 6, respectively) and isotype control goat IgG at 1 μg and 2 μg (lanes 7 and 8, respectively) were used. The arrow head indicates the TCDD-inducible DNA-protein complex. Results are representative of two separate experiments.