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. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2011 Feb 4;252(2):150–158. doi: 10.1016/j.taap.2011.01.020

Figure 7.

Figure 7

Knockdown of Bach2 mRNA in B cells using siRNA. B-cells were electroporated with an siRNA duplex targeting Bach2 (Bach2 siRNA), negative control siRNA duplexes (negative control siRNA and GL2 luciferase control), or mock electroporated (no siRNA duplexes). Following a 2 h recovery, cells were activated with LPS (10 μg/ml) and treated with either TCDD (10 nM) or DMSO (0.02%) for 48 h. Bach2 mRNA was measured using qRT-PCR. The data are presented as a percentage of the negative control siRNA duplex treated with LPS + DMSO. The bars are mean ± S.E. of triplicate experimental replicates.