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. 2011 Jun;21(6):908–914. doi: 10.1101/gr.116202.110

Figure 1.

Figure 1.

The hypermethylation–hypomethylation transition zone in the centromeric region of rice chromosome 11. (a) FISH mapping of the 5S rDNA on somatic metaphase chromosomes of rice. Arrows point to the single 5S rDNA locus (green) on rice chromosome 11. Bar, 5 μm. (b) Three-color FISH mapping of BAC clone 46A04 (green), 5S rDNA (red), and the CentO satellite repeat (blue) on meiotic pachytene chromosome 11 of rice. BAC 46A04 is the closest clone flanking the telomeric side of the 5S rDNA locus. Bar, 5 μm. (c) Immunodetection of 5mC on a super-stretched rice pachytene chromosome 11. The purple box includes the centromeric region, and the fluorescent signals in this region are exemplified and separated into different color channels. The 5S rDNA locus is located within the hypermethylation–hypomethylation transition zone. (d–f) Differential methylation along the 5S rDNA locus revealed by immunodetection of 5mC on DNA fibers. Approximately 41% of the 5S rDNA section toward the centromere is less methylated than the rest of the 5S rDNA array. Bars, 10 μm.