Figure 2.

Mapping of DNA methylation in the centromere of maize B chromosome. The positions of the five ZmB arrays (1–5) in the functional centromere are labeled. Fiber-FISH of ZmB (red) and CentC-CRM (the white signals were digitally converted from blue fluorescence signals) and detection of 5mC (green) were performed on the same DNA fiber. (Top panel) A diagram of the DNA composition of the maize B centromere. The functional B centromere spans ∼700 kb and contains five distinct arrays (1–5) of the ZmB repeat. The association of this 700-kb region with CENH3 was previously demonstrated by Jin et al. (2005). Each ZmB repeat array is flanked by intermingled CentC and CRM repeats.