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Temperature dependence of spin-lattice relaxation rates for dithionite reduced [4Fe-4S]+ in R. sphaeroides ETF-QO. The circles are the average values for wild type, N338T and N338A mutants. The error bars reflect the standard deviations for the three proteins. The fit line is the sum of contributions from the Raman and Orbach processes. The Debye temperature was fixed at 100 K and the energy of the low-lying excited state was ΔE = 210 K (146 cm−1).
