Table 1.
Location of Conserved Sequences in AHL-Lactonase and AHL-Acylase Genes of Taxonomically Diverse Bacterial Groups
| Taxonomic Group | Position (nts)a | Conserved Regions |
|---|---|---|
| AHL-lactonase gene | ||
| Allb | 467-484 | TGGTC[AG]AAATGCAGATGGGT |
| 557-571 | ATTTCGACCATGCCG | |
| 737-755 | AGCGATCACGCGTGTCC | |
| Actinobacteria | 560-570 | TCGACCACG[CG]CG |
| Firmicutes | 535-542 | TA[CT]ATTA |
| 559-566 | TTCGATCA | |
| 774-780 | ATATCGT | |
| Alphaproteobacteria | 562-571 | GACCAT[ACG]TCGT |
| Betaproteobacteria | 521-523 | CGG[AC]CG |
| 681-689 | CGCAGACCA | |
| Gammaproteobacteria | 527-534 | ATGTCGAG |
| 548-552 | CCCAT | |
| Deltaproteobacteria | 518-522 | A[GT]CCC |
| Euryarchaeota | 560-569 | T[CT]GACCACGC |
| Ascomycota | 338-347 | TTGAXCATCC |
| 520-539 | ATCAAAGACGTCAAGGCAGT | |
| 550-571 | CA[CT]TTACATCTCGA[CT]CACGCAG | |
| 628-633 | CACGAG | |
| AHL-acylase gene | ||
| Allc | 236-254 | TGATCGCCGAAATCCGGCG |
| 1892-1907 | GTATGGGTCCAGGCCA | |
| Actinobacteria | 704-713 | TACCGGGTCG |
| 1177-1181 | AC[AC]C[CG]GTCA[CG]C | |
| 1208-1217 | CCA[CG]GAGCTG | |
| Cyanobacteria | 1522-1527 | TTTGAAA |
| Alphaproteobacteria | 486-495 | TCGATGCG |
| Betaproteobacteria | 328-340 | GCGCAGGCAACC |
| Gammaproteobacteria | 309-320 | CGGCATCGGTTA |
| 586-595 | GGCTTCAACC | |
| Bacteroidetes | 1490-1497 | TC[CG]GCATA |
a:
Nucleotides in the gene from 5’ to 3’.
b:
Gene sequences from taxonomic groups listed under AHL-lactonase gene.
c:
Gene sequences from taxonomic groups listed under AHL-acylase gene.