Figure 5.

Inhibition of ¹²⁵I-labeled MGSA binding to K562 cells stably expressing the mouse Duffy-like protein and mouse erythrocytes. K562 cells and mouse erythrocytes were incubated for 30 min at 37°C with ¹²⁵I-labeled MGSA (0.5 nm) in the absence or presence of 0.25 μm of unlabeled MGSA, MCP-1, IL-8, RANTES, MIP-1α, MIP-1β, Eotaxin, GROα, and GROβ. The binding reactions were stopped as described (Neote et al. 1993; Chaudhuri et al. 1994). The data are expressed as cpm of ¹²⁵I-labeled MGSA bound (per 10⁶ cells) for K562 cells and (per 10⁷ cells) for mouse erythrocytes. Abbreviations for human and murine chemokines are hu and mu, respectively.