Table 2.
Sense strand | Antisense strand | ||||
---|---|---|---|---|---|
rawa | calibrated using | rawa | calibrated using | ||
Mb | M + Nc | Mb | M + Nc | ||
6393d | 6393.00 | 6393.00 | 6557d | 6557.00 | 6557.00 |
6474 | 6389.01 | 6393.03 | 6638 | 6556.32 | 6557.02 |
6457 | 6392.16 | 6392.98 | 6623 | 6560.09 | 6557.02 |
6476 | 6391.79 | 6393.01 | 6641 | 6560.12 | 6557.02 |
6469 | 6389.53 | 6392.01 | 6635 | 6557.35 | 6557.01 |
average | 6391.10 | 6392.81 | 6558.18 | 6557.01 | |
s.d.e | 1.55 | 0.40 | 1.61 | 0.01 |
Data points of peaks of R6G-labeled sense strand and R110-labeled antisense strand of PCR product of gyrA wild type examined by five successive runs.
Raw data without calibration.
Calibrated using size marker alone.
Calibrated using size marker and TAMRA-labeled normal PCR products.
Used as a template for calibration.
Standard deviation.