Table 4.

Statistical Evaluation of Mobility Shift

No.	Mobility shifta		No.	Mobility shifta
sense	antisense	sense	antisense
	gyrA			hFIX exon 7
1	−8.69	23.51	1	−11.62	−0.93
2	−11.21	−2.92	2	4.53	2.49
3	27.16	1.96	3	126.41	166.66
4	0.18b	11.18	4	−12.58	−2.08
5	53.21	−5.91	5	133.61	143.92
6	77.69	54.97
7	47.90	56.54
8	67.03	71.18		hFIX exon 8
9	−50.63	−24.51
10	−3.80	−1.95	1	11.13	52.11
11	−12.08	0.99	2	3.56	2.00
12	−4.83	−1.95	3	−55.12	−3.00
13	−3.80	14.11	4	15.43	32.93
14	−0.81b	0.01b	5	−41.01	−57.68c
15	−10.05	−2.89	6	−35.14	−57.34
16	1.22	4.09
17	2.20	4.02
18	72.58	79.07
19	78.01	75.30
20	93.44	79.23
21	28.89	0.99
22	8.15	12.37
23	1.19b	3.03

^aMobility shift is defined as a difference between data point of mutant peak and averaged data point of normal peak. The standard deviations of normal DNAs are gyrA sense strand, 0.40; gyrA antisense strand, 0.01; hFIX exon 7 sense strand, 0.46; hFIX exon 7 antisense strand, 0.10; hFIX exon 8 sense strand, 0.40; hFIX exon 8 antisense strand, 0.63. 

^bFalse negative (within 3× s.d. of normal DNA) 

^cA minor peak was observed at 0.04, presumably attributable to an isoconformer.