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Change in fragment size with increasing temperature. GS 2500 and 500 size standards were mixed and separated by use of condition POP-4b, with increasing temperatures (30°C–70°C). Changes in size for both strands of a fragment from GS 2500 are indicated. The known sequence-based sizes for the three fragments are 94 (A), 286 (B), and 470 nucleotides (C).
