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Matrix for the preparation of chondroitin sulfate standards for GAG assay
| CS-Ca (μg) | H2O (μl) | 0.2 mg/ml CS-C in H2O (μl) |
1 mg/ml CS-C in H2O (μl) |
|---|---|---|---|
| 0 | 75 | – | – |
| 1 | 60 | 15 | – |
| 2 | 45 | 30 | – |
| 3 | 30 | 45 | – |
| 4 | 15 | 60 | – |
| 5 | 60 | – | 15 |
| 6 | 57 | – | 18 |
This is the total amount of Chondroitin sulfate-C (CS-C) that is loaded in the wells; it is not a concentration
