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. Author manuscript; available in PMC: 2011 Jun 2.
Published in final edited form as: Mol Microbiol. 2009 Dec 4;75(2):440–451. doi: 10.1111/j.1365-2958.2009.06993.x

Fig. 3.

Fig. 3

Periplasmic OtpA(N′)-HA is not secreted by an OtpB-independent mechanism. HDB114 transformed with pPC11 (PBAD-otpB) and pPC110 [Ptrc-otpA(N′)-HA] were incubated with IPTG and then subjected to pulse-chase labelling. Arabinose was added at the beginning of the chase. Cultures were divided into cell (C) and secreted (S) fractions, and the cell fraction was divided into three portions. One aliquot was untreated, the second was treated with proteinase K (PK), and the third was converted to a spheroplast suspension prior to PK treatment (sph). Immunoprecipitations were conducted with the indicated antisera. The S samples were derived from three times as much culture volume as each C and sph sample. m, molecular weight markers.