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. Author manuscript; available in PMC: 2011 Jun 2.
Published in final edited form as: Mol Microbiol. 2009 Dec 4;75(2):440–451. doi: 10.1111/j.1365-2958.2009.06993.x

Fig. 6.

Fig. 6

A C-terminal disulphide bond reduces the efficiency of OtpA(N) secretion.

A. The location of the cysteine residues in OtpA(N)cys is shown.

B–C. HDB114 (B) or PC97 (C) transformed with pPC11 (PBAD-otpB) and pPC91 [Ptrc-otpA(N)cys] were incubated with IPTG and arabinose and then subjected to pulse-chase labelling. Cultures were divided into cell (C) and secreted (S) fractions and half of the cells were treated with proteinase K (PK). OtpA was immunoprecipitated from all samples. The S samples were derived from twice as much culture volume as each C sample. m, molecular weight markers.