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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Plant J. 2011 Jan 10;65(4):600–609. doi: 10.1111/j.1365-313X.2010.04448.x

Figure 5.

Figure 5

The N-terminal regions of pFtsH2 and pFtsH5 are able to insert into thylakoid membranes. Radiolabeled pFtsH2Δ or pFtsH5Δ (as diagrammed below the panels) was incubated with intact pea chloroplasts in a reaction containing 5mM ATP and light at 25 °C for 20 min. Intact chloroplasts were recovered (C) and treated with thermolysin (C+). Untreated intact chloroplasts were fractionated into stroma (S) and thylakoids. Thylakoid aliquots were washed with import buffer (T), treated with thermolysin (T+) or extracted with 100 mM NaOH (TN). For pFtsH5Δ, a thylakoid aliquot was extracted with 200 mM Na2CO3 (TCO). Samples were analyzed by SDS-PAGE on 12.5% gels and fluorography.