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Analytical HSCCC separation of the crude sample obtained from C. militaris after cleaning-up by 732 cation-exchange resin. Solvent system: n-hexane–n-butanol–methanol–water (23:80:30:155, v/v/v/v); stationary phase: upper phase; mobile phase: lower phase; flow rate: 1.0 ml/min; evolution speed: 1600 rpm; sample: 4.2 mg dissolved in 2 ml of the lower phase; retention of the stationary phase: 49%; detection wavelength: 254 nm. Peak c: cordycepin.
