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Preparative HSCCC separation of the crude sample obtained from C. militaris after cleaning-up by 732 cation-exchange resin. Solvent system: n-hexane–n-butanol–methanol–water (23:80:30:155, v/v/v/v); stationary phase: upper phase; mobile phase: lower phase; flow rate: 2.0 ml/min; revolution speed: 850 rpm; separation temperature: 30°; sample: 216.2 mg dissolved in 10 ml of the lower phase; retention percentage of stationary phase: 54%; detection wavelength: 254 nm. Peak C: cordycepin.
