Fig. 3.

Patients with glioblastoma (GBM) are deficient in IFN-γ production. Peripheral blood mononuclear cells from patients with GBM or healthy donors were stored in liquid N2, thawed, and rested overnight in complete media, and equal numbers of nonadherent cells were stimulated the following day with anti-CD3/CD28 antibody–coated beads and interleukin-2 for 72 h. CD3⁺ gated cells were analyzed for intracellular cytokine (ICC) staining by FACS. Columns in top panel represent mean percentage of interferon (IFN)–γ-positive cells. Live gated events are shown in the bottom panel.