Table 2.

Filter metric Assessment for Primer B4. Prior to optimization, zero trace files had a high quality filter metric (high quality TS and high quality CRL). After optimization, 222 trace files fit into the high quality category. Prior to optimization, 318 trace files require manual review and after primer specific filter metric settings were applied, only 97 trace files required manual review.

Assessment	Total no. of trace files before optimization	Total no. of trace files after optimization
High Quality	0	222
Review	318	97
Low Quality	34	33
TOTAL	352	352