Table 3.

Primer-specific optimized filter metrics. The values below are the primer specific filter metrics for each of the eight sequencing primers defined by our laboratory’s internal validation. These values should be used as initial settings for a laboratory. Internal validation should be performed by individual laboratories to define its laboratory-specific settings.

	Trace Score			Contiguous Read Length
Primer	Low Quality	Review	High Quality	Low Quality	Review	High Quality
R1	0-20	21-29	30-100	0-200	201-250	≥251
B1	0-20	21-27	28-100	0-150	151-210	≥211
C1	0-20	21-24	25-100	0-150	151-250	≥251
R2	0-20	21-24	25-100	0-110	111-250	≥251
A4	0-20	21-24	25-100	0-200	201-250	≥251
B4	0-20	21-24	25-100	0-150	151-200	≥201
C2	0-20	21-24	25-100	0-100	101-150	≥151
D2	0-20	21-24	25-100	0-100	101-150	≥151