Table 1.
Bacterial strains and plasmids
| Strains | genotype | origin |
|---|---|---|
| E. coli | ||
| DH5α | endA-1 hsdR-17 supE-44 thi-1 recA-1 gyrA relA-1 Δ(lacZYA-argG)U169 deoR | [57] |
| MT616 | MM294 pRK600 CmR | [58] |
| BL21(DE3) | F- dcm ompT hsd gal/λ(DE3) | [59] |
| Sinorhizobium meliloti | ||
| Rm1021 | SU47 SmR | [60] |
| R6.48 | Rm1021,ohrR::GmR | This study |
| R7.15 | Rm1021,ΔohrR ohr::GmR | This study |
| R7.16 | Rm1021,ohr+ ohrR+ ohr::lacZ ohrR::uidA | This study |
| R8.39 | Rm1021,ohr::GmR | This study |
| Plasmids | ||
| pGEMT | pUC derivative cloning vector, AmpR | Promega |
| pGEMTeasy | pUC derivative cloning vector, AmpR | Promega |
| pET22b+ | expression vector, AmpR | Novagen |
| pK18mobsacB | mobilisable pUC derivative, sacB NeoR | [51] |
| pBBR1-MCS2 | broad host range replicating mobilisable vector, NeoR | [61] |
| pBBR1-MCS5 | broad host range replicating mobilisable vector, GmR | [61] |
| pTH1505 | GmR, gfp, lacZ, uidA, rfp fusion vector | [54] |
| p34SGm | ori ColEI AmpR GmRcassette | [52] |
| pD3001 | pK18mobsacB (XbaI-PstI)/ohrR downstream region (XbaI-NsiI) | this study |
| pD3083 | pGEMTeasy/ ohrR upstream region | This study |
| pD4116 | pK18mobsacB ΔohrR | This study |
| pD4244 | pK18mobsacB ΔohrR::GmR | This study |
| pD5333 | pK18mobsacBΔohrR ohr::GmR | This study |
| pD5455 | pTH1505 ohr::lacZ, ohrR::uidA | This study |
| pD8657 | pK18mobsacB ohr::GmR | This study |
| pBBohr | pBBRI-MCS2 ohr+ | This study |
| pBBohrR | pBBRI-MCS2 ohrR+ | This study |
| pE1541 | pBBRI-MCS2 ohr::lacZ | This study |
| pETohrR | pET22b+ ohr+ | This study |