Figure 4.

Bazooka loss-of-function (LOF) clones in multiple lineages. Adult hemispheres showing Bazooka LOF MARCM clones induced at first instar and visualized with either (A-F) period-GAL4 (G,H) or atonal-GAL4. (A,B) Mosaic analysis with a repressible cell marker (MARCM) clones in the ventral projection neurons (vPNs) of the BAla1 lineage visualized with period-GAL4,UASGFP. (A) Control BAla1 secondary axon tracts only entered the middle antennal cerebral tract (mACT) (arrow). (B) BAla1 clones containing null alleles of bazooka were misguided into the mACT and inner (i)ACT (arrows). (C) control DALv2 clone with proximal arbors in the lateral triangle and IMP (arrow) and distal arbors in the central ellipsoid body (EB). (D) baz LOF clone in the DALv2 lineage with ectopic bulbous proximal projections (arrow). Inset shows a second DALv2 clone exhibiting a long bipolar-like neurite (arrowhead). (E) Control BAmv1 clone with distal projections into the fan-shaped body and inferior-medial protocerebrum (IMP) (arrow). (F) baz LOF clone with disorganized branching along the axon tract (double arrows) and abnormal distal arbors (arrow). Note smaller clone size in all baz LOF clones visualized with the period-GAL4 driver (arrowheads mark cell bodies). (G,G') BLD5 control clone visualized with atonal-GAL4 showing proximal projections into (G) the ipsilateral optic lobula and (G') about 11 distal branches in the contralateral optic medulla. (H,H') Baz LOF BLD5 clone visualized with atonal-GAL4 showing (H) ectopic projections into the ipsilateral medulla and (H') increased axonal extensions in the contralateral optic medulla. In all preparations, a single hemisphere is shown of an adult brain. Scale bars: 25 μm