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. 2011 Apr 27;6:16. doi: 10.1186/1749-8104-6-16

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Copyright ©2011 Spindler and Hartenstein; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Overexpression of Bazooka with period-GAL4 causes axon misguidance. Confocal sections of adult brains at the level of the ellipsoid body (EB) are shown with homozygous (A) period-GAL4,UAS-GFP or (B,D) homozygous period-GAL4,UAS-GFP;UAS-Baz. (D,E) Note that the BAla1 lineage (ventral projection neurons) made a loop before terminating in the lateral horn, and the EB failed to close ventrally (arrowhead). (C) Graph comparing the percentage of abnormal lineage morphology in the presence of single copies of UAS-mcd8GFP (n = 7) and Bazooka (n = 3) versus double copies of UAS-mcd8GFP (n = 6) and Bazooka (n = 6). Note that only lineages containing double copies of UAS-Bazooka showed defects. Scale bars: 15 μm