Figure 6. Dose-dependent rescue of the autophagy defect in brain of transgenic mice.

A) Western blots of accumulated autophagy markers LAMP2 and p62 and astrocyte marker GFAP in brain homogenates from wildtype, transgenic and Fig4 null mice at P28; 30 ug protein per lane. B) Accumulation of of LAMP2 and p62 immunofluorescence in GFAP-positive astrocytes in the cortex of transgenic lines at P28. Astrocyte abundance is dramatically increased in Fig4 null mice (Scale bars = 200 µm).