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. 2011 Jun 2;7(6):e1002106. doi: 10.1371/journal.pgen.1002106

Figure 3. cuoB is only up-regulated by copper and other divalent metals.

Figure 3

(A) The WT strain harboring the cuoB-lacZ fusion was spotted onto CTT agar plates containing metals or oxidants at the concentrations indicated above each picture. Plates also contained 5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside to qualitatively monitor β-gal activity (blue color development). Pictures were taken after 48 h of incubation. (B) Up-regulation of cuoB by Cd2+. The WT (continuous line) and the ΔcorE strains (dashed line) harboring the cuoB-lacZ fusion were incubated on CTT agar plates containing 0.1 mM Cd(NO3)2. β-gal specific activity was determined in cell extracts harvested at the indicated times. (C) Up-regulation of cuoB by Zn2+. The approach followed was the same as the one reported in panel B. The concentration of metal used was 0.4 mM Zn(NO3)2. Error bars indicate standard deviations.